High resolution fluorescent test chart

July 20, 2023

We have developed a high-resolution fluorescence test chart for microscopy and look forward to comments from the optics community! The first users particularly liked the fields with 20×20 pinholes. By this an microscope objective lens can be fully characterized with just one z-stack. Lines with a pitch down to 200 nm (5000 line pairs per mm) in horizontal and vertical orientation allow reaching the resolution limit of the best available oil immersion objectives. In addition to pure lens testing, it is possible to characterize entire systems such as laser scanning microscopes in terms of their resolution.
The test chart is fluorescent and can be excited between 420 and 520 nm. The emission is between 500 and 700 nm.

Download the data sheet from our test chart page.